ToulligQC report for MinION1D2_multiplex_FAH18855
Run id: MinION1D2_multiplex_FAH18855
Report name: 20170927_MinION1D2_multiplex_FAH18855
Run date: 2017-09-27T14:55:42Z
Report date : 11/19/20 15:07:53 CET

Summary

  1. Basic Statistics
  2. Read count histogram
  3. 1Dsquare read count histogram
  4. Read length histogram
  5. 1Dsquare read size histogram
  6. Yield plot of 1D read type
  7. Read type quality boxplot
  8. 1Dsquare reads quality boxplot
  9. Mean Phred score frequency of all 1D read type
  10. Mean Phred score frequency of 1Dsquare read type
  11. Mean Phred score function of 1D read length
  12. Mean Phred score function of 1Dsquare read length
  13. Channel occupancy of the flowcell
  14. 1D² Sequence length over time
  15. 1D² PHRED score over time
  16. 1D² Read speed over time
  17. Number of 1D² reads over time
  18. 1Dsquare pass reads percentage of different barcodes
  19. 1Dsquare fail reads percentage of different barcodes
  20. 1Dsquare read size distribution for each barcode
  21. 1Dsquare read phred score distribution for each barcode

Basic Statistics

Run info

MeasureValue
Run id MinION1D2_multiplex_FAH18855
Sample MinION1D2_multiplex_FAH18855
Report name 20170927_MinION1D2_multiplex_FAH18855
Run date 2017-09-27T14:55:42Z
Run duration 47:59:58
Flowcell id FAH18855
Flowcell version FLO-MIN107
Kit SQK-LSK308
Yield (Gbp) 2.05
Read count 1825754
N50 (bp) 1478

Software info

MeasureValue
MinKNOW version 1.7.14
Basecaller name guppy-basecalling
Basecaller version 2.3.5+53a111f
Basecaller analysis 1dsqr_basecalling
ToulligQC version 2.0b2
Hostname dnacpc14
Device Unknown
Device ID MN17734
Model file template_r9.5_450bps_5mer_raw.jsn

Read count 1D pass 1D fail
count 1825754 1202729 623025
frequency 100.00 65.88 34.12

Read count 1D² Read count 1D² pass 1D² pass barcoded 1D² fail 1D² fail barcoded
count 1825754 221424 2553 2546 218987 166082
frequency 100.00 100.00 1.15 1.15 98.90 75.01

1D 1D pass 1D fail
count 1825754 1202729 623025
mean 7.36 8.51 5.14
std 1.93 0.85 1.43
min 1.00 7.00 1.00
25% 6.27 7.84 4.29
median 7.81 8.48 5.46
75% 8.79 9.15 6.33
max 12.07 12.07 7.00

1D² 1D² pass 1D² fail
count 221540 2553 218987
mean 6.88 11.31 6.83
std 2.26 0.26 2.22
min 0.00 11.00 0.00
25% 5.28 11.11 5.27
median 6.87 11.24 6.81
75% 8.73 11.45 8.68
max 12.63 12.63 11.00

1D 1D pass 1D fail
mean 7.36 8.51 5.14
std 1.93 0.85 1.43
min 1.00 7.00 1.00
25% 6.27 7.84 4.29
median 7.81 8.48 5.46
75% 8.79 9.15 6.33
max 12.07 12.07 7.00

1D² 1D² pass 1D² fail
mean 6.88 11.31 6.83
std 2.26 0.26 2.22
min 0.00 11.00 0.00
25% 5.28 11.11 5.27
median 6.87 11.24 6.81
75% 8.73 11.45 8.68
max 12.63 12.63 11.00

Channel occupancy of the flowcell 🉣

Channel occupancy of the flowcell image

barcode arrangement 1D² read count
barcode01 19.11% 488
barcode02 25.03% 639
barcode03 12.46% 318
barcode04 15.43% 394
barcode05 14.06% 359
barcode07 13.63% 348
other 0.04% 1
unclassified 0.24% 6

barcode arrangement 1D² read count
barcode01 14.35% 31302
barcode02 17.10% 37303
barcode03 10.49% 22879
barcode04 11.39% 24842
barcode05 10.77% 23500
barcode07 12.04% 26256
other 0.61% 1338
unclassified 23.26% 50742

1D 1D pass 1D fail
qscore qscore qscore
count 221424 2553 218871
mean 6.88 11.31 6.83
std 2.26 0.26 2.22
min 0.00 11.00 0.00
25% 5.29 11.11 5.27
50% 6.87 11.24 6.82
75% 8.73 11.45 8.68
max 12.63 12.63 11.00

Produced by ToulligQC (version 2.0b2)